Adaptation to Tissue Culture of the Murine Leukemia ASL1, a High Producer of TL (Thymus Leukemia) Antigen1
- 1 October 1975
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 6 (4) , 335-341
- https://doi.org/10.1111/j.1399-0039.1975.tb00652.x
Abstract
ASL1, a. murine thymus derived leukemia, was adapted to tissue culture and its suitability as a source of TL antigens was examined. Tissue culture medium was developed in which the cell grew with a mean doubling time of 12.5 h and 95% viability. The quantity of antigen expressed by the cells was determined by inhibition of lysis of 51Cr labelled leukemic cells mediated by anti-TL 1,2,3 serum. Significant quantities of the antigens were retained by the cells during exponential growth in vitro.Keywords
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