GROWTH AND HABITUATION IN TISSUE CULTURES OF ENGLISH IVY, HEDERA HELIX

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Abstract
Tissue cultures from both juvenile and adult stems of English ivy, Hedera helix L., were established in White's medium supplemented by coconut water and auxin (usually naphthalene acetic acid). With repeated transfers, cultures were habituated in less than a year to grow well without coconut water by using an auxin and kinetin. Cultures from juvenile seedlings were less demanding in requirements for growth. In all types of cultures occasionally small areas of rapidly growing cells were noticed. These when isolated gave rise to rapidly growing cultures with many cells of unusual appearance. Abnormally long cells and chain‐type cells were abundant. When 0.1 mg/ liter of kinetin was added to the medium, these cells grew well without auxin and coconut water.