Japanese encephalitis virus nonstructural protein NS3 has RNA binding and ATPase activities
- 1 January 1995
- journal article
- Published by Springer Nature in Virus Genes
- Vol. 9 (2) , 105-112
- https://doi.org/10.1007/bf01702653
Abstract
Sequence data suggest that Japanese encephalitis virus (JEV) protein NS3 is a multifunctional protein with sequence motifs characteristic of a protease and a helicase. To examine the functions of JEV-NS3, a fusion protein of NS3 inEscherichia coli was generated. Analysis by Western blot using monospecific rabbit antisera generated against the fusion protein (anti-MBJEN3) showed that NS3 was localized in the membrane fraction of JEV-infected cells and the particulate fraction of bacteria extracts. The addition of anti-MBJEN3 sera reduced JEV-specific RNA synthesis activity in a in vitro system. In addition, NS3 was shown to exhibit RNA binding and ATPase activities, suggesting this protein has an important role in viral RNA replication in virus-infected cells.Keywords
This publication has 36 references indexed in Scilit:
- Monoclonal antibodies identify the NS5 yellow fever virus non-structural protein in the nuclei of infected cellsJournal of General Virology, 1992
- Detection of a trypsin-like serine protease domain in flaviviruses and pestvirusesVirology, 1989
- In vitro synthesis of Japanese encephalitis virus (JEV) RNA: membrane and nuclear fractions of JEV-infected cells possess high levels of virus-specific RNA polymerase activityVirus Research, 1989
- A new superfamily of replicative proteinsNature, 1988
- The nucleotide sequence of dengue type 4 virus: Analysis of genes coding for nonstructural proteinsVirology, 1987
- Nucleotide sequence at the 3′ end of Japanese encephalitis virus genomic RNAVirology, 1986
- Nucleotide Sequence of Yellow Fever Virus: Implications for Flavivirus Gene Expression and EvolutionScience, 1985
- Replication strategy of Kunjin Virus: Evidence for recycling role of replicative form RNA as template in semiconservative and asymmetric replicationVirology, 1985
- Association of Virus Specific Replicative Ribonucleic Acid with Nuclear Membrane in Chick Embryo Cells Infected with Japanese Encephalitis VirusJournal of General Virology, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970