Evidence against VIP as the inhibitory transmitter in non‐adrenergic, non‐cholinergic nerves supplying the longitudinal muscle of the mouse colon

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Abstract
1 Vasoactive intestinal peptide (VIP) had two types of effects on the longitudinal muscle of the mouse distal colon. 2 At low concentrations (10−8 M) VIP induced a contraction which seemed to be related to the production of prostaglandins as it was abolished after preincubation with indomethacin (10−6 M). 3 At higher concentrations (3 × 10−8 and 10−7 M) VIP induced relaxations which developed slowly and were related to stimulation of the adenylate cyclase activity of the smooth muscle cells. 4 There is no evidence that VIP is the non-adrenergic, non-cholinergic transmitter released by electrical stimulation in this preparation and responsible for rapid relaxation of the smooth muscle.