Autoradiography of Mast Cells Incubated With Tritiated Histidine, Cytidine or Thymidine

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Abstract
Rat peritoneal fluid was incubated in vitro after addition of tritiated histidine, cytidine or thymidine. Histidine H3 was added alone, in combination with pyridoxal phosphate, or in combination with pyridoxal phosphate, chloramphenicol and ethiomine. Brush preparations of these various incubates were then coated with Kodak NTB3 emulsion for autoradiography. Most mast cells became labeled (in the cytoplasm) after incubation with histidine (control), more so in presence of pyridoxal phosphate, and greater than control, although reduced, labeling still occurred when chloramphenicol and ethionine also were present. Incubation with cytidine (RNA precursor) led to labeling of the nucleus (short term incubation), the labeling becoming cytoplasmic following longer incubation times; almost all mast cells were labeled. With thymidine (DNA precursor) only a small proportion of mast cells became labeled (in the nucleus). These evidences of high and varied biochemical activity are discussed with reference to mast cell cytology and to whether they can mitose once fully differentiated.

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