Further evaluation of indirect immunofluorescence methods for detection of antibodies againstAspergillus fumigatus

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Abstract
The IF titres of IgG antibodies to hyphal antigen preparations of 6 isolates of Aspergillus fumigatus were compared with the titres to germling antigen preparations of the same isolates. Titres varied according the antigen used and the method of preparation. No single isolate or method of antigen preparation enabled a group of 15 patients with aspergillosis to be separated from a group of asthmatic subjects or a group of patients with other fungal infections, but significantly higher mean IF titres were obtained for the group of patients with aspergillosis.