PRP16 is an RNA-dependent ATPase that interacts transiently with the spliceosome
- 7 February 1991
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 349 (6309) , 494-499
- https://doi.org/10.1038/349494a0
Abstract
The assembly of the spliceosome is an ATP-dependent process. The splicing factor PRP16 contains variations of several motifs that define the elF-4A-like ATP-dependent RNA helicase family. The protein has now been purified and shown to exhibit RNA-dependent ATPase activity. PRP16 is required specifically for the second catalytic step of the splicing reaction in vitro. This function requires ATP binding and/or hydrolysis, which appears to be concomitant with release of the protein from the spliceosome. PRP16 may be the prototype for a set of splicing factors which use ATP to drive a cycle of conformational changes.Keywords
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