Identification of H‐2 and Ia‐Antigen Analogues in Several Species by Immunological Crossreactions of Xenoantisera

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Abstract
Rabbit antisera against H-2K and D antigens react with molecules composed of 12,000 and 45,000 dalton subunits derived from human, monkey, rat, guinea-pig, cow and pig lymphocytes. The antisera failed to react with similar type molecules from chicken. A rabbit anti-human .beta.2-microglobulin serum reacted with chicken molecules comprising 12,000 and 45,000 dalton polypeptide chains. An antiserum against HLA-DR antigens reacted with Ia-[immune response associated]-antigen-like molecules from monkey, rat, mouse, guinea-pig, cow, pig and chicken lymphocytes. All Ia-antigen-like molecules displayed 2 dissimilar subunits. Xenoantisera directed against highly purified MHC [major histocompatibility complex] antigens from 1 sp. may be useful tools in elucidating the structure of similar antigens in other species where alloantisera are not available.