Glycopeptide Composition of Hepatitis B Surface Antigen
- 1 May 1980
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 48 (1) , 31-38
- https://doi.org/10.1099/0022-1317-48-1-31
Abstract
Summary Three major polypeptides of hepatitis B surface antigen (HBsAg), with mol. wt. 22000 (p22), 27000 (p27) and 68000 (p68), were separated by preparative SDS-PAGE. These three peptides as well as intact HBsAg were found to have almost identical amino acid compositions and carbohydrate was detected in p27 and p68 by PAS staining. Papain treatment of p68 produced two distinct peptides, p27 and p22. Moreover, when an artificial mixture of p27 and p22 in a ratio of 1:1 was treated with 0.2 m-periodate for 30 min at 37 °C, only p22 was detectable. These results suggest that p68 is composed of p27 and p22, and that p27 is a glycosylated product of p22. Thus, from the evidence obtained, it is possible that p22 (22000 peptide) is the minimum size of the unique hepatitis B virus (HBV) gene product involved.This publication has 1 reference indexed in Scilit:
- Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen.Proceedings of the National Academy of Sciences, 1977