ENHANCEMENT OF RADIORESPONSE OF A MOUSE MAMMARY-CARCINOMA TO COMBINED TREATMENTS WITH HYPERTHERMIA AND RADIOSENSITIZER MISONIDAZOLE

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (8) , 2966-2970
Abstract
The regrowth delay of a transplanted syngeneic mouse mammary carcinoma designated MT2 was used to estimate the effects of 3-fold combination treatments: X-irradiation; hyperthermia and radiosensitizer; and misonidazole (Ro-07-0582). The experiment entailed 5 groups of experimental mice: untreated control; X-rays alone; X-rays plus hyperthermia (42-43.degree. C); X-rays plus misonidazole; and X-rays plus hyperthermia plus misonidazole. X-ray treatments consisted of 4000 rads administered locally to the tumors in 2 equal fractions at a 48 h interval. Misonidazole (0.67 mg/g body wt) was injected i.p. 30 min before exposure to X-irradiation. Hyperthermia was administered 10 min prior to and for 17 min during irradiation. The regrowth delay factor of 3.9 was obtained by administering combined treatments of the 3 agents (X-rays plus hyperthermia plus misonidazole). The enhancing effect on a syngeneic mouse mammary adenocarcinoma by the modality of treatment herein described lends support to the usefulness of combining X-rays with other agents in the treatment of neoplasms.