Primary structure of cathepsin D inhibitor from potatoes and its structure relationship to soybean trypsin inhibitor family
- 17 July 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 251 (1-2) , 94-98
- https://doi.org/10.1016/0014-5793(89)81435-8
Abstract
A novel effective procedure for the purification of cathepsin D inhibitor from potatoes (PDI) was developed. The amino acid sequence of PDI was determined by analysis of the cyanogen bromide digest and of the limited tryptic and chymotryptic digests of the protein. The inhibitor is a single polypeptide chain protein consisting of 188 residues with a simple sugar moiety attached to Asn‐19. The tentative disulfide pairings are also suggested. The sequence data clearly indicate that PDI is homologous with the soybean trypsin inhibitor (STI) (Kunitz) family. The active center of PDI for trypsin inhibition was identified as Pro‐Val‐Arg‐Phe in analogy to STI.Keywords
This publication has 28 references indexed in Scilit:
- On the size of the active site in proteases. I. PapainPublished by Elsevier ,2005
- Age-related increase in a cathepsin D-like protease that degrades brain microtubule-associated proteinsBiochemistry, 1987
- The primary structure of calf thymus glutaredoxin. Homology with the corresponding Escherichia coli protein but elongation at both ends and with an additional half-cystine/cysteine pairEuropean Journal of Biochemistry, 1984
- Chemical characterization of cathepsin D inhibitor from potatoesCollection of Czechoslovak Chemical Communications, 1977
- Secondary Structures of Proteins from the 30S Subunit of theEscherichia coliRibosomeHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1977
- Further characteristics of cathepsin D inhibitor from potatoesCollection of Czechoslovak Chemical Communications, 1976
- Isolation and some properties of cathepsin D inhibitor from potatoesCollection of Czechoslovak Chemical Communications, 1976
- Crystal structure of the complex of porcine trypsin with soybean trypsin inhibitor (Kunitz) at 2.6 Å resolutionBiochemistry, 1974
- Effect of pepsin inhibitor from Ascaris lumbricoides on cathepsin D and EBiochimica et Biophysica Acta (BBA) - Enzymology, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970