A Radioimmunoassay for 1,25-Dihydroxycholecalciferol

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Abstract
1. 1α,25-Dihydroxy-25-hemisuccinate cholecalciferol has been synthesized and conjugated to bovine serum albumin. 2. This conjugate is immunogenic; when injected into rabbits antibodies of high affinity for 1α,25-dihydroxycholecalciferol were obtained. 3. Vitamin D metabolites lacking the 1α-hydroxy group were of lower cross-reactivity with the antibodies. 4. By using these antibodies and 1α,25-[23,24-3H]dihydroxycholecalciferol as tracer a sensitive radioimmunoassay has been developed capable of detecting 20 pg of 1α,25-dihydroxycholecalciferol.