Biosynthesis of δ-Aminolevulinic Acid from Glutamate in Agmenellum quadruplicatum

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Abstract
δ-Aminolevulinic acid accumulated in the culture medium when Agmenellum quadruplicatum strain PR-6 was incubated in the presence of levulinic acid, a competitive inhibitor of δ-aminolevulinic acid dehydratase, and specifically labeled glutamate and glycine. The δ-aminolevulinic acid was purified using Dowex 50W-X8 and cleaved by periodate to yield succinic acid and formaldehyde. The distribution of radioactivity in the two fragments suggested that in blue-green algae the carbon skeleton of δ-aminolevulinic acid is derived directly from glutamate. However the possibility of the pathway of δ-aminolevulinic acid synthesis, from glycine and succinyl-coenzyme A also functioning in blue-green algae was not eliminated as uptake of glycine was minimal.
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