Stability of Repetitive-Sequence PCR Patterns with Respect to Culture Age and Subculture Frequency
Open Access
- 1 June 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (6) , 2694-2696
- https://doi.org/10.1128/jcm.41.6.2694-2696.2003
Abstract
To examine the stability of repetitive-sequence (rep) PCR profiles, six species of bacteria were subcultured to blood agar plates and DNA was extracted from the cultures after 24, 48, and 72 h of incubation at 35°C. In addition, the same species were subcultured to fresh blood plates daily and DNA was extracted from the cultures after growth of 5, 10, and 15 subcultures, respectively. rep PCR analysis demonstrated that all rep PCR fingerprints from a single species were identical.Keywords
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