The formation of bacterial proteases, especially in synthetic media

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Abstract
Sterile filtrates of broth cultures of Bacillus mesentericus and Pseudomonas liquefied commercial gelatin and digested commercial caseinogen beyond the acid-coagulable stage. Purified egg-albumin was resistant to the enzymes of these bacteria. They grew well on Merrill and Clark''s single medium if mixtures of salts of Ca and Mg were present or if Mg salts were added. Ca salts alone had very little effect on their growth. Ca salts alone or a mixture of Ca and Mg salts influenced the liberation of protease in simple synthetic medium. Very little protease formation occurred in presence of Mg alone, even though there had been good growth. Ca and Mg salts stimulated protease formation in a medium containing ammonium lactate and asparagine.

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