Ethanol-Potassium Nitrate Medium for Enumerating Rhizoctonia solani-like Fungi from Soil
- 1 January 1987
- journal article
- research article
- Published by Scientific Societies in Plant Disease
- Vol. 71 (12) , 1098-1100
- https://doi.org/10.1094/pd-71-1098
Abstract
A medium consisting of 0.2 g of potassium nitrate, 30 g of agar, 947 ml of distilled water, 53 ml of 95% ethyl alcohol, 0.38 ml of metalaxyl 2E, 0.02 ml of prochloraz 40EC, 100 mg of tobramycin, and 300 mg of streptomycin was developed for enumerating Rhizoctonia solani-like fungi from soil. Ethyl alcohol was mixed with the cooled medium at 50 C before antibiotics and fungicides were added. Selectivity decreased with age of the medium, thus freshly made media should be used. Most colony-forming units of R. solani-like fungi in field soils were present in organic matter and retained by a 65-mesh sieve when soil suspensions were screened. A few colony-forming units collected on a 200- or 270-mesh sieve were microsclerotia and resting hyphae free of organic matter.Keywords
This publication has 1 reference indexed in Scilit:
- A selective medium for isolating Rhizoctonia solani from soilAnnals of Applied Biology, 1985