Production of YY Male in the Guppy Poecilia reticulata by Endocrine Sex Reversal and Progeny Testing

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Abstract
Gravid Poecilia reticulata were orally administered estrogens, b-estradiol (200- 400 mgkg-1 food), diethylstilbestrol (100-1,000 mgkg-1 food), 17 a-ethynylestradiol (100- 500 mgkg-1 food) and 8-benzoate estradiol (200-800 mgkg-1 food) from days 15 to 20 after the previous parturition until the termination of gestation. Treatment with 400 mg 11,-estradiol per kg food, 300 mg diethylstilbestrol per kg food and 200 mg 1 7 a-ethynylestradiol per kg food, initiated 5-10 days prior to parturition, caused the birth of 100% females. However, S.benzoate estradiol, even if administered at high dosage levels, failed to induce 100% sex reversal. Sex-reversed females were functional and their genotype was identified by progeny testing. Sex-reversed females (XY} were mated with normal males (XY) to produce F 2 progeny. Among 21 F 2 males, each tested individually by meting with a normal female (XX), two males were identified to have YY genotype and both of them sired all-male offspring. A program for the mass production of YY broodstock is discussed.

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