Oxygen uptake by entrapped hybridoma cells

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Abstract
Oxygen consumption by hybridoma cells immobilized in 1- and 3.9-mm-diameter calcium alginate beads was measured. The entrapped cells consumed oxygen at about 10 μmol/min per 109 cells, regardless of the bead size and cell loading. In contrast, the same cells in suspension culture respire at specific rates of 3–8 μmol/min per 109 cells (depending on the cell density). The growth rate of the immobilized cells was significantly reduced, while specific antibody production was comparable to that of free cells.