Separation of MET-tRNAf- and MET-tRNAmby Chromatography On Sepharose 4B Columns
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 6 (5) , 339-346
- https://doi.org/10.1080/00327487608061623
Abstract
Unfractionated rabbit liver tRNA, charged with [3H]methionine by use of rat liver enzymes, was separated into two [3H]methionine-containing fractions by column chromatography on Sepharose 4B. The two fractions were identified as Met-tRNAm Met and Met-tRNAf met by (a) their different ability to form a GTP- -dependent ternary complex with IF-MP, and (b) the absence of the first fraction after selective charging of the tRNA with E. coli amino acyl tRNA synthetase. The methionine residue was without noticeable influence on the separation.This publication has 12 references indexed in Scilit:
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