Human Immunodeficiency Virus Type I Provirus Is Demonstrated in Peripheral Blood Monocytes In Vivo: A Study Utilizing an In Situ Polymerase Chain Reaction
- 1 January 1993
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 9 (1) , 69-76
- https://doi.org/10.1089/aid.1993.9.69
Abstract
Human immunodeficiency virus type 1 (HIV-1) infects a variety of cell types in vivo. Monocyte/macrophages may be the major reservoir for HIV-1 in the solid tissues of HIV-1-infected individuals. Conflicting data have been reported, though, regarding the presence of HIV-1 provirus in peripheral blood monocytes isolated from HIV-1-seropositive humans. We have evaluated monocytes from the peripheral blood of eleven HIV-1-infected individuals utilizing a new, highly sensitive and specific in situ polymerase chain reaction. We demonstrate HIV-1 provirus in 73% (8/11) of these samples. None of these monocyte samples was demonstrated to contain cells expressing high levels of HIV-1-specific RNA, by standard in situ hybridization. The evaluation of the HIV-1 genome in peripheral blood monocytes of certain infected individuals may assist in the understanding of HIV-1 proviral latency and pathogenesis, in vivo.Keywords
This publication has 56 references indexed in Scilit:
- Rapid In Situ Detection of PCR-Amplified HIV-1 DNADiagnostic Molecular Pathology, 1992
- In Situ Polymerase Chain Reaction Detection of Viral DNA, Single-Copy Genes, and Gene Rearrangements in Cell Suspensions and CytospinsDiagnostic Molecular Pathology, 1992
- Macrophage and T cell-line tropisms of HIV-1 are determined by specific regions of the envelope gp!20 geneNature, 1991
- HIV-1 tropism for mononuclear phagocytes can be determined by regions of gp120 outside the CD4-binding domainNature, 1990
- Specific Tropism of HIV-1 for Microglial Cells in Primary Human Brain CulturesScience, 1990
- Interleukin 6 induces human immunodeficiency virus expression in infected monocytic cells alone and in synergy with tumor necrosis factor alpha by transcriptional and post-transcriptional mechanisms.The Journal of Experimental Medicine, 1990
- Infection of the Retina by Human Immunodeficiency Virus Type INew England Journal of Medicine, 1987
- Pathogenesis of Infection with Human Immunodeficiency VirusNew England Journal of Medicine, 1987
- Monocyte function in the acquired immune deficiency syndrome. Defective chemotaxis.Journal of Clinical Investigation, 1984
- Decreased expression of human class II antigens on monocytes from patients with acquired immune deficiency syndrome. Increased expression with interferon-gamma.Journal of Clinical Investigation, 1984