A Genetic System for Trichothecene Toxin Production inGibberella pulicaris (Fusarium sambucinum)

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Abstract
Trichothecene-toxin-producing isolates of Gibberella pulicaris (anamorph: Fusarium sambucinum) were mated on water agar containing steam-sterilized mulberry (Morus alba) twigs. Mature ascospores developed at 15 C following several weeks of incubation under an alternating 12 hr/25 C light and 12 hr/20 C dark schedule. Segregation patterns among random ascospore progeny from parents differing in diacetoxyscirpenol production and other traits were examined. Protoperithecium formation and red pigmentation were monogenically controlled. Preliminary analyses of progency from crosses among toxin-producing and nonproducing isolates indicated that ability to produce toxin is controlled by genes at several loci. We conclude that trichothecene toxin biosynthesis in G. pulicaris is amenable to classical genetic analysis. This is the first documented genetic system in a trichothecene-producing fungus.

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