Synthesis of citrovorum factor in rats given chloretone

Abstract
The effect of ascorbic acid on increased citrovorum-factor synthesis is shown in vivo with chloretonized rats, which elaborate increased amounts of ascorbic acid. The observations were extended by a study of the in vivo conversion of intraperitoneally administered pteroylglutamic acid into citrovorum factor by normal and chloretonized animals. From a study of the effect of chloretone feeding on liver-choline-oxidase, xanthine-oxidase and tyrosine-oxidase activities, it is concluded that the direct effects of the narcotic on the animal are probably far greater than those attributable to increased conversion of pteroylglutamic acid into citrovorum factor.