Four gene products are required for the fungal synthesis of the indole‐diterpene, paspaline
- 17 February 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (6) , 1625-1630
- https://doi.org/10.1016/j.febslet.2006.02.008
Abstract
Paspaline belongs to a large, structurally and functionally diverse group of indole-diterpenes synthesized by filamentous fungi. However, the identity of the gene products required for the biosynthesis of paspaline, a key intermediate for the synthesis of paxilline and other indole-diterpenes, is not known. Transfer of constructs containing different pax gene combinations into a paxilline negative deletion derivative of Penicillium paxilli demonstrated that just four proteins, PaxG, a geranylgeranyl diphosphate synthase, PaxM, a FAD-dependent monooxygenase, PaxB, a putative membrane protein, and PaxC, a prenyl transferase, are required for the biosynthesis of paspaline.Keywords
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