Inhibition of nucleoside diphosphate kinase (NDPK/nm23) by cAMP analogues
- 2 January 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 400 (1) , 75-79
- https://doi.org/10.1016/s0014-5793(96)01358-0
Abstract
Nucleoside diphosphate kinase (NDPK/nm23) ATP/GDP phosphotransferase activity and serine autophosphorylation is inhibited by N 6‐mbcAMP, 8‐ClcAMP and 8‐BrcAMP. Inhibition of the enzymatic activity largely depends on the concentration of ATP and becomes significant at ATP concentrations up to 0.5 mM and at effector concentrations measured in C6 cells stimulated with 1 mM cAMP analogue. N 6‐mbcAMP is a substrate of the enzyme. DbcAMP and O′2‐mbcAMP, cAMP analogues with a modified O′2‐ribose, did not affect the NDPK activity. Cyclic AMP is only a moderate inhibitor of NDPK even at low ATP concentrations. Possible inhibitory effects of cAMP and cAMP analogues on reported extra‐ and intracellular functions of NDPK/nm23 are discussed.Keywords
This publication has 38 references indexed in Scilit:
- Myocardial Glucose Metabolism and ATP Levels Are Decreased Two Days after Global IschemiaJournal of Surgical Research, 1996
- Biochemical modulation of tumor cell energy IV: Evidence for the contribution of adenosine triphosphate (ATP) depletion to chemotherapeutically-induced tumor regressionBiochemical Pharmacology, 1995
- The 1.9 Å Crystal Structure of a Nucleoside Diphosphate Kinase Complex with Adenosine 3′, 5′-Cyclic Monophosphate: Evidence for Competitive InhibitionJournal of Molecular Biology, 1995
- Physiological and pathological roles of purines: An updateDrug Development Research, 1993
- Statistische Prämienkalkulation nach dem Exponentialprinzip bei unbekannter RisikoverteilungBlätter der DGVFM, 1992
- Identity of a differentiation inhibiting factor for mouse myeloid leukemia cells with NM23/nucleoside diphosphate kinaseBiochemical and Biophysical Research Communications, 1992
- Transphosphorylation and G protein activationBiochemical Pharmacology, 1990
- Generation of extracellular atp in blood and its mediated inhibition of host weight loss in tumor-bearing miceBiochemical Pharmacology, 1989
- A very fast ion-pair reversed-phase HPLC method for the separation of the most significant nucleotides and their degradation products in human red blood cellsAnalytical Biochemistry, 1987
- Transient increase in intracellular concentration of adenosine 3′:5′‐cyclic monophosphate results in morphological and biochemical differentiation of C6 glioma cells in cultureJournal of Neuroscience Research, 1987