Glucose decreases respiratory control ratio in EL‐4 tumor cells
- 23 November 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 313 (2) , 126-128
- https://doi.org/10.1016/0014-5793(92)81427-n
Abstract
EL-4 ascites thymoma cells are shown to have high aerobic glycolysis and decreased Pasteur effect. At the same time, glucose produces a much smaller inhibitory effect on cell respiration (Crabtree effect) than in Ehrlich ascites carcinoma (EAC) cells. In intact EL-4 cells, the respiratory control ratio (RCR) was found to be 6.2 with endogenous substrates and 8.0 with glutamine. Glucose decreased the RCR to 3.2, by stimulating, the state 4 respiration. In rat thymocytes and EAC cells, such an effect of glucose was absent (RCR of 7.0 and 7.2, respectively). It is suggested that in EL-4 tumor cells, the high aerobic glycolysis and small Crabtree effect may be due to glucose-induced ‘uncoupling’ of oxidation and phosphorylationKeywords
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