Transcription of the α2u-globulin gene in male rat liver nuclei in vitro
- 25 July 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (15) , 3092-3096
- https://doi.org/10.1021/bi00608a023
Abstract
.alpha.2u-Globulin is a male rat liver protein under multihormonal control which represents approximately 1% of hepatic protein synthesis. The rate of transcription of the .alpha.2u-globulin gene was measured using a nuclear cell-free transcriptional system with mercurated CTP as substrate for the endogenous RNA polymerases. The newly synthesized, mercurated RNA was purified free of endogenous RNA by chromatography on sulfhydryl agarose and hybridized to 3H-labeled .alpha.2u-globulin cDNA. It was found that, in male rat liver nuclei, .alpha.2u-globulin RNA sequences represent 0.005% of the total newly synthesized RNA. Actinomycin D or .alpha.-amanitin completely blocks the synthesis of .alpha.2u-globulin RNA in these nuclei. No .alpha.2u-globulin RNA synthesis was detectable in female rat liver nuclei. Thus, within the limits of the detection the absence of hepatic .alpha.2u-globulin mRNA in female rats appears to be due to a lack of transcription of the .alpha.2u-globulin gene in these animals.This publication has 1 reference indexed in Scilit: