Free cytoplasmic Ca ++ at rest and after cholinergic stimulus is increased in cultured muscle cells from Duchenne muscular dystrophy patients
- 1 March 1988
- journal article
- research article
- Published by Wolters Kluwer Health in Neurology
- Vol. 38 (3) , 476
- https://doi.org/10.1212/wnl.38.3.476
Abstract
We used a fluorescent dye, quin 2, to measure intracellular free calcium ([Ca+ +]i) in cultured skeletal muscle cells and skin fibroblasts from five Duchenne muscular dystrophy (DMD) patients and from five controls. We observed an enhanced [Ca+ +]i level, at rest and after acetylcholine (ACh) stimulation, in DMD muscle cells, but we did not detect any difference between DMD and normal skin fibroblasts. The abnormally higher [Ca+ +]i transient induced by ACh suggests that it plays a critical role in muscle degeneration. The skin fibroblast results suggest that there is no generalized membrane defect.This publication has 5 references indexed in Scilit:
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