[3H]5‐Hydroxytryptamine Binding to Brain Astroglial Cells: Differences Between Intact and Homogenized Preparations and Mature and Immature Cultures

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Abstract
[3H]5‐hydroxytryptamine ([3H]serotonin) binds with high affinity (KD 2–12 nM) to a finite number of sites on brain astroglial cells. The number of binding sites in the C6 glioma line is decreased significantly (Bmax= 315 versus 30 fmol/mg) by homogenization. In intact primary cultures, derived from newborn rat brain, the number of binding sites is far greater in cultures of immature astrocytes than in cultures treated with dibutyryl cyclic AMP (Bmax= 1,520 versus 580 fmol/mg). A role for these receptors in development is suggested.