Oxygen evolving membranes and particles from the transformable cyanobacterium Synechocystis sp. PCC6803
- 1 November 1989
- journal article
- research article
- Published by Springer Nature in Photosynthesis Research
- Vol. 22 (2) , 123-130
- https://doi.org/10.1007/bf00035442
Abstract
Membranes and PS II particles retaining high rates of O2-evolving activity have been isolated from the transformable cyanobacterium, Synechocystis sp. PCC6803. Membranes from cells grown under red light exhibit rates of O2-evolution ranging from 500–700 μmole O2/mg chl/h. PS II particles are prepared by a simple procedure involving DEAE column chromatography of detergent extracts obtained by simultaneous treatment of membranes with octylglucoside and dodecylmaltoside. The isolated PS II fraction is enriched in polypeptides immunologically cross-reactive with polypeptides present in core reaction center preparations of spinach, exhibits 77 K fluorescence emission maxima at 685 and 696 nm, but not emission and absorption due to phycobilines and is capable of rates of O2-evolution exceeding 1000 μmole O2/mg chl/h.Keywords
This publication has 18 references indexed in Scilit:
- Purification and properties of an oxygen-evolving Photosystem II reaction-center complex from spinachBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1987
- A reevaluation of the fluorescence of the core chlorophylls of Photosystem IBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1986
- An oxygen‐evolving complex with a simple subunit structure ‐ ‘a water‐plastoquinone oxidoreductase” ‐ from the thermophilic cyanobacteriumSynechococcussp.FEBS Letters, 1985
- A Highly Active Oxygen-Evolving Photosystem II Preparation from the Cyanobacterium Anacystis nidulansPlant Physiology, 1984
- Purification of Photosystem II particles from Phormidium laminosum using the detergent dodecyl-β-d-maltoside. Properties of the purified complexBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1983
- Isolation of oxygen‐evolving phycobilisome—photosystem II particles from Porphyridium cruentumFEBS Letters, 1983
- Chlorophyll-protein organization of membranes from the cyanobacterium Anacystis nidulansArchives of Biochemistry and Biophysics, 1983
- A rapid method for extraction of oxygen‐evolving photosystem 2 preparations from cyanobacteriaFEBS Letters, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- ON SPECTRAL CONTROL OF PIGMENTATION IN ANACYSTIS NIDULANS (CYANOPHYCEAE)1,2Journal of Phycology, 1978