Evidence for the attachment of Hsp150/Pir2 to the cell wall ofSaccharomyces cerevisiaethrough disulfide bridges
- 1 December 2001
- journal article
- Published by Oxford University Press (OUP) in FEMS Yeast Research
- Vol. 1 (3) , 241-245
- https://doi.org/10.1111/j.1567-1364.2001.tb00040.x
Abstract
Here we present evidence that Hsp150/Pir2, a member of the Pir family of cell wall proteins, can be extracted from the purified cell walls of Saccharomyces cerevisiae by treatment with beta-mercaptoethanol, demonstrating that at least part of this protein is attached to the cell wall through disulfide bridges. We also present evidence that Pir4, another member of this family, is partly secreted to the growth medium. Finally we propose a hypothesis to explain the relationship between the differently localized forms of particular members of the Pir family of cell wall proteins.Keywords
This publication has 17 references indexed in Scilit:
- Cell wall dynamics in yeastCurrent Opinion in Microbiology, 1999
- The contribution of the O‐glycosylated protein Pir2p/Hsp150 to the construction of the yeast cell wall in wild‐type cells and β1,6‐glucan‐deficient mutantsMolecular Microbiology, 1999
- New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiaeYeast, 1994
- Review: Cell wall assembly in yeastYeast, 1994
- Is there a role for GPIs in yeast cell-wall assembly?Trends in Cell Biology, 1994
- A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein.Proceedings of the National Academy of Sciences, 1992
- [12] One-step gene disruption in yeastPublished by Elsevier ,1983
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970