CHANGES IN PHOSPHOLIPID MOLECULAR-SPECIES COMPOSITION OF SOYBEAN HYPOCOTYL AND COTYLEDON AFTER DEDIFFERENTIATION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 531  (1) , 25-31
Abstract
The effect of dedifferentiation on the molecular species composition of soybean [Glycine max] phospholipids was studied by using hypocotyl, cotyledon and the suspension culture cells established from those organs. Three major phospholipids (phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol) and phosphatidylmonomethylethanolamine were composed of 12 molecular species. Major species were 1-palmitoyl-2-linoleoyl, 1-oleoyl-2-linoleoyl, 1-palmitoyl-2-linolenoyl and 1-linoleoyl-2-linoleoyl species. Different proportions of the molecular species were found among the 3 major phospholipids, but phosphatidylmonomethylethanolamine was composed of the same proportions of the molecular species as those of phosphatidylethanolamine. After dedifferentiation, the 1-palmitoyl-2-linoleoyl species increased in the cells established from hypocotyl. In the cells established from cotyledon, the 1-palmitoyl-2-linolenoyl species increased dramatically. In both cells, the 1-palmitoyl-2-linolenoyl species increased in response to increase in the 2,4-D concentrations and the progress of cell growth.