Simian Virus 40 -- Chinese Hamster Kidney Cell Interaction. IV. Enhanced Virus Replication in Infected Cells upon Treatment with Mitomycin C

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Abstract
Chinese hamster kidney cells are semi-permissive to SV-40. Exposure to mitomycin C (MC) of Chinese hamster kidney cells infected with SV-40 DNA enhanced the yield of infectious virus 10- to 100-fold. This stimulation occurred whether the treatment was performed before or after infection. A simultaneous increase in the number of [virus capsid] V antigen-synthesizing cells and virus-producing cells, and the virus burst size, was observed on MC pretreatment, but the proportion of T [tumor] antigen-synthesizing cells remained unchanged. MC pretreatment clearly stimulated virus DNA replication in SV-40-virus-infected cells. Cells treated with MC exhibited an unbalanced growth pattern, with continuing protein synthesis in the absence of cell division and a markedly reduced ability to replicate the cellular DNA. MC probably enhances the permissiveness of Chinese hamster kidney cells by inducing the synthesis of a specific cellular factor(s) required for SV-40 replication in these cells. Exposure to UV light also enhanced infectious virus production in Chinese hamster kidney cells.

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