ACTIVATION OF SCHRADAN BY MAMMALIAN TISSUE HOMOGENATES

1 January 1956

journal article
Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology

Vol. 34 (1) , 1131-1141
https://doi.org/10.1139/y56-117

Abstract

It has been confirmed that the combined microsomal and soluble fractions of liver, in the presence of magnesium, nicotinamide, and DPN, can convert schradan to a potent anticholinesterase. DPN can be replaced by TPN, but not by ATP. DPNH can replace the DPN and soluble fraction. Catalase enhances the conversion but only when DPNH or a source of it is provided. Acetone powders of microsomes, suitably fortified, are almost ineffective in converting schradan. The pH optimum of the whole schradan-converting system is 8.1. Other properties of the system are described. Certain extrahepatic tissues, especially lung, heart, and testis, can convert schradan. The livers of all species tested convert schradan. A peroxide-mediated mechanism of oxidation is proposed.

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