Differentiation of 3T3-L2 fibroblasts into adipose cells in bromodeoxyuridine-suppressed cultures.
- 1 September 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (9) , 4451-4454
- https://doi.org/10.1073/pnas.76.9.4451
Abstract
Growth of 3T3-L2 fibroblasts in medium containing 5-bromo-2'-deoxyuridine prevents the spontaneous cytodifferentiation of these cells into adipose cells. Treatment of bromodeoxyuridine-suppressed cultures at confluence with 1-methyl-3-isobutylxanthine, a promoter of the adipose conversion, results in differentiation of the fibroblasts into adipose cells but the extent of the conversion is less than that seen in cultures not treated with bromodeoxyuridine. 1-Methyl-3-isobutylxanthine appears to function without altering the amount of bromodeoxyuridine present in DNA.Keywords
This publication has 20 references indexed in Scilit:
- Studies of lipoprotein lipase during the adipose conversion of 3T3 cellsCell, 1978
- Deoxycytidine reverses the suppression of pigmentation caused by 5-BrdUrd without changing the amount of 5-BrdUrd in DNACell, 1977
- Alterations in insulin binding accompanying differentiation of 3T3-L1 preadipocytes.Proceedings of the National Academy of Sciences, 1977
- Acquisition of increased hormone sensitivity during in vitro adipocyte development.Journal of Biological Chemistry, 1977
- Conversion of 3T3 fibroblasts into adipose cells: triggering of differentiation by prostaglandin F2alpha and 1-methyl-3-isobutyl xanthine.Proceedings of the National Academy of Sciences, 1976
- Selective interaction of 5'-bromodeoxyuridine substituted DNA with different chromosomal proteinsBiochemistry, 1976
- Induction of lipogenesis during differentiation in a "preadipocyte" cell line.Journal of Biological Chemistry, 1976
- An established preadipose cell line and its differentiation in culture II. Factors affecting the adipose conversionCell, 1975
- Toward Molecular Mechanisms of Developmental ProcessesAnnual Review of Biochemistry, 1973
- Preferential inhibition by 5-bromodeoxyuridine of the synthesis of tyrosine aminotransferase in hepatoma cell culturesJournal of Molecular Biology, 1971