A quantitative selected ion monitoring assay for ketoprofen determination in human biological fluids

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Abstract
A new very sensitive method of ketoprofen [(benzoyl-3-phenyl)2-propionic acid] determination in various biological fluids using selected ion monitoring is described. After ether extraction in acidic medium, ketoprofen and (benzoyl-4-phenyl)-2-butyric acid used as internal standard are methylated and their concentrations determined by monitoring the m/z values corresponding to their respective molecular ions. Standard deviation is below 5% at 100 ng ml−1. Detection limits range from 10 ng ml−1 for urine and serum samples to 50 ng ml−1 for maternal milk specimens. This method has been applied successfully to various biological studies: pharmacokinetic profiles, bioavailability and transfer of the drug through physiological barriers.

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