Determination of Monensin Sodium Residues in Beef Liver Tissue by Liquid Chromatography of a Fluorescent Derivative
- 1 November 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 68 (6) , 1149-1153
- https://doi.org/10.1093/jaoac/68.6.1149
Abstract
Monensin sodium does not have an ultraviolet (UV) absorbance above 220 nm, and therefore cannot be detected by liquid chromatography (LC) with a UV detector. A method was developed in which monensin residues are extracted from beef liver tissue, acetylated, partitioned, and reacted with 9-anthryldiazomethane to form a fluorescent derivative for quantitation by LC. The reliable level of sensitivity is 50 ppb, but 15 pjib can be detected. Recoveries ranged between 71 and 96% with an average of 83.5%.This publication has 2 references indexed in Scilit:
- Identification and Semiquantitation of Monensin Sodium in Animal Feeds by Thin Layer BioautographyJournal of AOAC INTERNATIONAL, 1983
- 9-Diazomethylanthracene as a new fluorescence and ultraviolet label for the spectrometric detection of picomole quantities of fatty acids by high-pressure liquid chromatographyAnalytical Biochemistry, 1980