The Interaction of Clostridium perfringens and Its Toxins in the Production of Necrotic Enteritis of Chickens

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Abstract
The intraduodenal administration of large numbers of C. perfringens cells harvested from broth cultures and resuspended in phosphate buffered saline or fresh sterile thioglycollate broth produced a very mild form of necrotic enteritis. Administering an appropriate number of cells in culture supernatant, however, produced typical field-type disease. The significant toxin recoverable from broth-culture supernatant fluids was .alpha.-toxin. Requirements to produce the disease are minor intestinal damage and sufficient numbers of toxigenic C. perfringens in the intestine.