A Study of the Malpighian Tubules of the Pill Millipede, Glomeris Marginata (Villers)

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Abstract
1. The Malphigian tubules of the pill millipede, Glomeris marginata are described. 2. After the necessary analyses of the haemolymph had been carried out a Ringer solution was prepared which would support the secretion of tubule fluid in vitro. 3. The reasons for the variation and deterioration in the secretion rates of control tubules are discussed. The presence of various amino acids in the bathing medium appears to be essential for fluid production. 4. There would not appear to be a diuretic hormone involved in the control of secretion; and 5-HT, cylic AMP and theophylline will not stimulate fluid production. 5. Secretion is inhibited by the metabolic inhibitors 2,4-DNP, azide and cyanide. The relative insensitivity shown to the latter two compounds is probably associated with a resistance mechanism.