Insulin receptor overexpression in 184B5 human mammary epithelial cells induces a ligand‐dependent transformed phenotype
- 1 April 1995
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 57 (4) , 666-669
- https://doi.org/10.1002/jcb.240570411
Abstract
To determine the role of the insulin receptor overexpression in breast epithelial cell transformation, the 184B5 human breast epithelial cell line was transfected with human insulin receptor cDNA. In two cell lines transfected with and overexpressing human insulin receptors (IR) (223.8 and 184.5 ng IR/106 cells), but not in untransfected cells, insulin binding and tyrosine kinase activity were elevated, and insulin induced a dose-dependent increase in colony formation in soft agar.Keywords
This publication has 13 references indexed in Scilit:
- Phosphatidylinositol-3-kinase is a non-tyrosine phosphorylated member of the insulin receptor signalling complexBiochemical and Biophysical Research Communications, 1992
- Overexpression of Insulin Receptors in Fibroblast and Ovary Cells Induces a Ligand-Mediated Transformed PhenotypeMolecular Endocrinology, 1991
- Elevated insulin receptor content in human breast cancer.Journal of Clinical Investigation, 1990
- Overexpression of the human insulinlike growth factor I receptor promotes ligand-dependent neoplastic transformation.Molecular and Cellular Biology, 1990
- A Diffuse Infiltrative CD8 Lymphocytosis Syndrome in Human Immunodeficiency Virus (HIV) Infection: A Host Immune Response Associated with HLA-DR5Annals of Internal Medicine, 1990
- Isolation and growth of human mammary epithelial cellsJournal of Tissue Culture Methods, 1985
- Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo[a]pyrene.Proceedings of the National Academy of Sciences, 1985
- Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract.Proceedings of the National Academy of Sciences, 1984
- Insulin Secretion in ObesityNew England Journal of Medicine, 1967
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949