The Nutrition of Streptococcus salivarius

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Abstract
A chemically defined medium has been devised which will support the growth of Str. salivarius cultures. The simplest effective medium contained, in addition to inorganic salts, glucose and Na thioglycollate, the following substances: glutamic acid, leucine, arginine, iso-leucine, lysine, methionine, tyrosine, riboflavin, nicotinic acid, pantothenic acid, biotin. thiamin and uracil. No other streptococci were found that would grow in the medium with the exception of a few cultures of other spp. within the Str. viridans group. The necessity of heating the medium in order to obtain satisfactory growth response was noted, together with a possible explanation of this effect.