Tricarboxylic Acid Cycle Mutants in Saccharomyces: Comparison of Independently Derived Mutants

Abstract
A yeast mutant independently isolated as a glutamate auxotroph ( glt 2-1 ) was similar to the glt 1-1 mutant in exhibiting a blocked tricarboxylic acid cycle due to the lack of aconitate hydratase. The new mutant differed by exhibiting blocks in lysine and cytochrome biosynthesis which segregated together with the glutamate requirement.