The akuB KU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus
Top Cited Papers
- 1 January 2006
- journal article
- research article
- Published by American Society for Microbiology in Eukaryotic Cell
- Vol. 5 (1) , 207-211
- https://doi.org/10.1128/ec.5.1.207-211.2006
Abstract
To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuBKU80). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuBKU80 mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuBKU80 had no influence on pathogenicity in a low-dose murine infection model.Keywords
This publication has 27 references indexed in Scilit:
- Gene Targeting in Aspergillus fumigatus by Homologous Recombination Is Facilitated in a Nonhomologous End- Joining-Deficient Genetic BackgroundEukaryotic Cell, 2006
- Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatusNature, 2005
- Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungiFungal Genetics and Biology, 2004
- Rapid Production of Gene Replacement Constructs and Generation of a Green Fluorescent Protein-Tagged Centromeric Marker in Aspergillus nidulansEukaryotic Cell, 2004
- Gene silencing with RNA interference in the human pathogenic fungus Aspergillus fumigatusFEMS Microbiology Letters, 2004
- Gene silencing with RNA interference in the human pathogenic fungus Aspergillus fumigatusFEMS Microbiology Letters, 2004
- Deletion of the Aspergillus fumigatus lysine biosynthesis gene lysF encoding homoaconitase leads to attenuated virulence in a low-dose mouse infection model of invasive aspergillosisArchiv für Mikrobiologie, 2004
- New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiaeYeast, 1994
- Regulation of the mRNA levels of nimA, a gene required for the G2-M transition in Aspergillus nidulans.The Journal of cell biology, 1987
- Meiotic and Mitotic Recombination in Aspergillus and Its Chromosomal AberrationsAdvances in Genetics, 1977