Effects of Donor and Host Lymphoid and Myeloid Tissue Injections in Lethally X-irradiated Mice Treated with Rat Bone Marrow23

Publisher Website
Google Scholar
Abstract
The effect of injections of isologous mouse or rat spleen, thymus, and bone marrow on the response of lethally X-irradiated LAF1 mice treated with rat bone marrow was investigated. The injection of isologous mouse spleen or thymus homogenate on the day of irradiation or 12 days after lethal irradiation with 870 r results in the rapid rejection of the rat bone-marrow graft and death due to bone-marrow failure. No adverse effect upon the survival time of irradiated mice and persistence of the rat bone-marrow graft is noted when isologous liver homogenate, lysed isologous liver, lysed isologous spleen, and lysed isologous thymus are injected. The injection of isologous mouse bone marrow on the day of irradiation or 3 days after irradiation results in the rejection of the rat bone-marrow graft and survival times as long as those seen in irradiated mice treated with isologous bone marrow alone. The survival times of lethally X-irradiated mice treated with bone marrow are significantly shortened when rat spleen or thymus is injected with the rat bone marrow. The rat bone-marrow graft in these mice continues to proliferate until their death. Death results, presumably, from an immunological response of the injected rat cells. No adverse effect either on the graft or on survival time of the host is noted when lysed or lyophilized rat tissue is given with rat bone marrow. The significance of these findings in elucidating some of the immunological principles involved in foreign bone-marrow transplantation is discussed.