Detection of differential sensitivity to 5‐fluorouracil in ehrlich ascites tumour cells by 19F NMR spectroscopy
- 1 December 1991
- journal article
- research article
- Published by Wiley in NMR in Biomedicine
- Vol. 4 (6) , 274-278
- https://doi.org/10.1002/nbm.1940040605
Abstract
Quantitative analysis of extracts from two Ehrlich ascites tumour cell lines (Lettre cells) by 19F NMR in vitro demonstrated that one Lettre cell line (designated LLM) metabolized 30-50% less 5-fluorouracil to 5-fluoronucleotides when compared to the other cell line (designated LHM). HPLC analysis of these cellular extracts showed a significant decrease in the concentration of the cytotoxic nucleotide 5-fluorouridine triphos-phate in LLM cells compared to LHM cells. No major differences could be observed in the 31P and 1H NMR spectra of the two cell lines. Growth inhibition studies in vitro demonstrated that LLM cells were less sensitive to 5-fluorouracil than LHM cells. These results are consistent with the hypothesis that 19F NMR visible levels of 5-fluoronucleotides can predict the cytotoxicity of the anti-cancer drug 5-fluorouracil.Keywords
This publication has 17 references indexed in Scilit:
- Chemotherapy-induced changes in the energetics of human breast cancer cells; 31P- and 13C-NMR studiesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990
- 19F MRS studies of fluoropyrimidine chemotherapy. A reviewNMR in Biomedicine, 1989
- Direct 19f NMR spectroscopic observation of 5‐fluorouracil metabolism in the isolated perfused mouse liver modelNMR in Biomedicine, 1988
- Lactic dehydrogenase isozymes, 31P magnetic resonance spectroscopy, and in vitro antimitochondrial tumor toxicity with gossypol and rhodamine-123.Journal of Clinical Investigation, 1987
- Phosphorus-31 nuclear magnetic resonance spectroscopic observation of the intracellular transformations of oncostatic cyclophosphamide metabolitesJournal of Medicinal Chemistry, 1986
- NMR study of in vivo RIF-1 tumorsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1984
- Proton Nuclear Magnetic Resonance of Intact Friend Leukemia Cells: Phosphorylcholine Increase During DifferentiationScience, 1982
- Phosphate metabolites in lymphoid, Friend erythroleukemia, and HeLa cells observed by high-resolution 31P nuclear magnetic resonance.Proceedings of the National Academy of Sciences, 1978
- 31P nuclear magnetic resonance studies of Ehrlich ascites tumor cells.Proceedings of the National Academy of Sciences, 1977