Isolation, Purification, and Some Properties of Two Viruses from Cultivated Cymbidium Orchids

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Abstract
[long dash]Two distinct viruses have been isolated from cultivated Cymbidium plants in Australia. Each virus isolate has been transmitted to herbaceous plants, has been purified, and high-titre antiserum has been produced. One isolate has been identified as a serotype of tobacco mosaic virus; the other is suspected of being Cymbidium mosaic virus and has flexuous rods about 13 by 475 m[mu]. Electron micrographs of particles of the latter show that they have hollow central canals and consist of helical structures with a pitch of approximately 2.8 m[mu]. Results indicate that inoculation to herbaceous plants and electron-microscopic examination and serological testing of leaf extracts can be used as methods for the routine indexing of Cymbidium plants.

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