Cell-Associated Virions and Nucleoids of a Murine Leukemia Virus2

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Abstract
Cell-associated nucleoids and virions were identified in the cytoplasm of established mouse cell lines infected with a murine leukemia virus. The buoyant density of the cell-associated virions ranged from 1.14–1.16 g/cm3,- buoyant densities of cell-associated nucleoids were 1.22–1.24 g/cm3 after cen-trifugation through potassium citrate gradients. Cell-associated virions and nucleoids were isolated from potassium citrate gradient fractions that did not contain ribosomal RNAs. The cell-associated virions and nucleoids were distinguished from polysomes and mitochondria by their resistance to disruption by ethylene-diaminetetraacetic acid (EDTA) and RNAse. Cell-associated virions and nucleoids incorporated3H-uridine within 10 minutes of exposure to label. Actinomycin D inhibited de novo incorporation of3H-uridine into these structures within 20 minutes, but did not inhibit the maturation of previously labeled cell-associated virions and nucleoids into extracellular virions. Cell-associated virions contained 2 RNA species with sedimentation coefficients of 36 S and 20–24 S.

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