Some Properties of Alkaline Phosphatase of Pseudomonas fluorescens
- 1 April 1967
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 1 (2) , 193-198
- https://doi.org/10.1111/j.1432-1033.1967.tb00063.x
Abstract
The formation of alkaline phosphatase in a strain of Pseudomonas fluorescens was found to be induced by limiting quantities of orthophosphate in the extracellular medium. The substrate specificity of this enzyme seemed to be similar to that of Escherichia coli. On the other hand, it had some properties different from those of the E. coli enzyme. The Pseudomonas enzyme was several hundred fold more sensitive to inhibition by EDTA, but was not inhibited by cyanide and by various mercaptans. The apparent Km for p‐nitrophenylphosphate was 2.0x10−4 M and Ki for orthophosphate as a competitive inhibitor was 3.7 × 10−5 at pH 8.6.This publication has 47 references indexed in Scilit:
- ELECTRON MICROSCOPY OF ALKALINE PHOSPHATASE OF ESCHERICHIA COLICanadian Journal of Microbiology, 1966
- Isolation and characterization of complementation products of Escherichia coli alkaline phosphataseJournal of Molecular Biology, 1966
- Excretion of alxaline phosphatase by Bacillus subtilisBiochemical and Biophysical Research Communications, 1964
- Mechanisms of Certain Phosphotransferase Reactions: Correlation of Structure and Catalysis in some Selected EnzymesAnnual Review of Biochemistry, 1964
- The amino acid sequence around the reactive serine in alkaline phosphataseBiochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1963
- Complementation in vivo between structural mutants of alkaline phosphatase from E. coliJournal of Molecular Biology, 1963
- Hybrid protein formation of E. coli alkaline phosphatase leading to in vitro complementationJournal of Molecular Biology, 1963
- The localization of alkaline phosphatase in E., coli K12Biochemical and Biophysical Research Communications, 1961
- A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. Coli I. Purification and characterization of alkaline phosphataseBiochimica et Biophysica Acta, 1960
- Fe++-dependent alkaline phosphatase of yeastBiochimica et Biophysica Acta, 1959