CELLULAR UPTAKE OF HYDROXYETHYLVINYLDEUTEROPORPHYRIN(HVD) AND PHOTOINACTIVATION OF CULTIVATED HUMAN LEUKEMIA (REH6) CELLS

Publisher Website
Google Scholar
Abstract
Abstract—Thein vitroincorporation of purified hydroxyethylvinyldeuteroporphyrin (HVD) into cells (Reh6) derived from an acute lymphocytic human leukemia is investigated using quantitative extraction techniques and fluorescence spectroscopy. A fast incorporation step (< 2 min) is characterized by its dependence on the porphyrin concentration in the incubation medium which suggests a saturation process. It is followed by a slower uptake, the rate of which linearly depends on the porphyrin concentration. No preferential uptake of aggregated form of HVD, which is shown to dimerize with an equilibrium constant of 9.7 × 105M−1, can be evidenced. As inferred from fluorescence spectra of cell suspensions and those of HVD dissolved in aqueous and micellar solutions as references, the porphyrin is mainly located in membrane structures and to a lower extent in cytoplasm. Cell photoinactivation does not depend on the incubation time but is only related to the intracellular porphyrin concentration.