Randomly picked cosmid clones overlap thepyrB andoriC gap in the physical map of theE.colichromosome
Open Access
- 25 March 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (6) , 2601-2612
- https://doi.org/10.1093/nar/16.6.2601
Abstract
Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyr B(96.5′) and ori C (84′) in the published physical map of E.coli [7]. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.Keywords
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