A Rapid Enzymatic Assay for Methotrexate in Serum
- 1 January 1979
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 17 (4) , 235-239
- https://doi.org/10.1515/cclm.1979.17.4.235
Abstract
A kinetic enzymological assay for methotrexate [an antineoplastic agent] in human serum is described; the results are available after 1.5 h. The lower limit of sensitivity is 50 nmol/l; the day-to-day variation coefficients compare favorably with other procedures (about 7% in the 200-600 nmol/l range). The method uses purified dihydrofolic acid reductase [EC 1.5.1.3] from bovine liver. The problem of its instability in the assay is circumvented by several measures, e.g., the use of a reaction rate analyzer.This publication has 13 references indexed in Scilit:
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